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Since the primary electron current lost directly to the anode is generally minimized for best efficiency, the discharge current is carried to the anode mainly by the plasma electrons. The plasma electrons are almost exclusively lost at the magnetic cusps, but their motion is affected by the presence of ions that also penetrate the cusp. Therefore, ions and electrons are lost to a hybrid anode area [27] at the cusp given by A, = 4 rh L, = 4

15-11.1.1 Mixing by Simultaneous Feed. Mixing using two or more feeders represents a continuous mixing process. Metering each ingredient precisely and bringing the streams together blends the components. For free- owing solids, a static mixer can be used. There is little chance for backmixing or axial mixing

frequency criteria, design is complete. If necessary, repeat this process one additional 1 in. increment. If more than 1 in. of additional diameter over the 2 calculated diameter for strength is required to meet the natural frequency criterion, go to the next step. 5. Select a hollow shaft, usually a standard pipe size, that meets the mechanical stress requirements [eqs. (21-7) and (21-8)]. Compute the critical speed

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Timeline and events in the discovery of anaerobic methane oxidation (AMO): from eld geochemistry to metagenomics 1 ~1957 Chemical oceanographers speculate Data sets from vertical sediment pro les show that methane and sulfate are simultaneously depleted in discrete zones in vertical sediment pro les 2 1979 Recognition of thermodynamic potential for CH4 serving as e donor Early laboratory incubations led to the hypothesis methanogenesis working in reverse in conjunction with a sulfate reducer However, robust enrichment cultures are unsuccessful (Zehnder and Brock, 1979) 3 1999 Use of eld biogeochemistry and gene cloning Sediments above a methane hydrate deposit in the coastal Paci c Ocean have a lipid biomarker with a 13C/12C value that proves it was derived from the methane deposit on the ocean oor The methane must have been the carbon source for the microorganisms.

Clone libraries of 16S rRNA genes reveal several unusual sequences that suggest the identity of organisms involved in AMO (Hinrichs et al, 1999) 4 2000 Fluorescent in situ hybridization (FISH) microscopy and radiotracer based physiology Microscopy (FISH) of eld samples proves robust physical associations between cooperating methanogenic Archaea and sulfate-reducing Bacteria Laboratory assays show that 35SO4 is reduced to H235S (Boetius et al, 2000) 5 2001 Con rmation of 13C/12C biomarker and FISH Spatially resolved mass spectrometry con rms that the eld-derived associations of methanogens and sulfate reducers are composed of carbon whose 13C/12C ratio links them to in situ methane oxidation (Orphan et al, 2001) 6 2002 Con rmation of biogeochemical signi cance: AMO creates large geochemical deposits in Black Sea.

Large reefs (deposits of Ca/MgCO3) at the bottom of the Black Sea were created from the CO2 produced by AMO; evidence is supported by FISH, conversion of 14CH4 to 14CO2, and stable isotope ratios in both lipid biomarkers and the carbonate minerals (Michaelis et al, 2002) 7 2003 Field enzymology Extracted protein from Black Sea reefs reveals an abundant nickelcontaining enzyme that is closely related to enzymes in methanogenic Archaea (Kruger et al, 2004) 8 2004 Community genomics (metagenomic) approach develops preliminary genetic blueprint for biochemical and genetic basis of process DNA sequences from shotgun and fosmid libraries (see Section 69) are assembled from a partially puri ed microbial community from the coastal Paci c Ocean Partial assembly of genome fragments from two archaeal genomes and three sulfate reducers result.

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Identi cation of most of the genes associated with methanogenesis provides strong support for the reverse methanogenesis hypothesis (Hallam et al, 2004)..

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